High efficiency transformation of maize (Zea mays L...

来自 : 发布时间：2024-05-07

AbstractTransformants of maize inbred A188 were efficiently produced from immature embryos cocultivated with Agrobacterium tumefaciens that carried 鈥渟uper-binary鈥?vectors. Frequencies of transformation (independent transgenic plants/embryos) were between 5% and 30%. Almost all transformants were normal in morphology, and more than 70% were fertile. Stable integration, expression, and inheritance of the transgenes were confirmed by molecular and genetic analysis. Between one and three copies of the transgenes were integrated with little rearrangement, and the boundaries of T-DNA were similar to those in transgenic dicotyledons and rice. F1 hybrids between A188 and five other inbreds were transformed at low frequencies. Subscription info for Chinese customersWe have a dedicated website for our Chinese customers. 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Rep. 5: 387鈥?05.CAS聽 Article聽Google Scholar聽 Download referencesAuthor informationAuthor notesTakashi KumashiroPresent address: Agribusiness Division, Japan Tobacco Inc., 2-1 Toranomon 2-chome, Minato-ku, Tokyo, 105, JapanYuji Ishida: KYR03634@niftyserve.or.jpAffiliationsPlant Breeding and Genetics Research Laboratory, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka, 438, JapanYuji Ishida,聽Hideaki Saito,聽Shozo Ohta,聽Yukoh Hiei,聽Toshihiko Komari聽 聽Takashi KumashiroAuthorsYuji IshidaView author publicationsYou can also search for this author in PubMed聽Google ScholarHideaki SaitoView author publicationsYou can also search for this author in PubMed聽Google ScholarShozo OhtaView author publicationsYou can also search for this author in PubMed聽Google ScholarYukoh HieiView author publicationsYou can also search for this author in PubMed聽Google ScholarToshihiko KomariView author publicationsYou can also search for this author in PubMed聽Google ScholarTakashi KumashiroView author publicationsYou can also search for this author in PubMed聽Google ScholarRights and permissionsReprints and PermissionsAbout this articleCite this articleIshida, Y., Saito, H., Ohta, S. et al. High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens. Nat Biotechnol 14, 745鈥?50 (1996). https://doi.org/10.1038/nbt0696-745Download citationReceived: 29 December 1995Accepted: 28 February 1996Issue Date: 01 June 1996DOI: https://doi.org/10.1038/nbt0696-745 Masoumeh Asadi-Aghbolaghi, Beata Dedicova, Sonali Sachi Ranade, Kim-Cuong Le, Farzad Sharifzadeh, Mansoor Omidi Ulrika Egertsdotter Plant Methods (2021) Joanna Melonek, Jorge Duarte, Jerome Martin, Laurent Beuf, Alain Murigneux, Pierrick Varenne, Jordi Comadran, Sebastien Specel, Sylvain Levadoux, Kalia Bernath-Levin, Fran莽ois Torney, Jean-Philippe Pichon, Pascual Perez Ian Small Nature Communications (2021) Albert P. Kausch, Kimberly Nelson-Vasilchik, Michael Tilelli Joel P. Hague In Vitro Cellular Developmental Biology - Plant (2021) Sign up for the Nature Briefing newsletter 鈥?what matters in science, free to your inbox daily.