High efficiency transformation of maize (Zea mays L...

来自 : 发布时间：2024-12-25

AbstractTransformants of maize inbred A188 were efficiently produced from immature embryos cocultivated with Agrobacterium tumefaciens that carried 鈥渟uper-binary鈥?vectors. Frequencies of transformation (independent transgenic plants/embryos) were between 5% and 30%. Almost all transformants were normal in morphology, and more than 70% were fertile. Stable integration, expression, and inheritance of the transgenes were confirmed by molecular and genetic analysis. Between one and three copies of the transgenes were integrated with little rearrangement, and the boundaries of T-DNA were similar to those in transgenic dicotyledons and rice. F1 hybrids between A188 and five other inbreds were transformed at low frequencies. Subscription info for Chinese customersWe have a dedicated website for our Chinese customers. Please go to naturechina.com to subscribe to this journal.Go to naturechina.comRent or Buy articleGet time limited or full article access on ReadCube.from$8.99Rent or BuyAll prices are NET prices. References1De Cleene, M. and Deley, J. 1976. The host range of crown gall. Bot. Rev. 42: 389鈥?66.Article聽Google Scholar聽 2Grimsley, N., Hohn, T., Davies, J.W. and Hohn, B. 1987. Agrobacterium-mediated delivery of infectious maize streak virus into maize plants. Nature 325: 177鈥?79.CAS聽 Article聽Google Scholar聽 3Gould, J., Devey, M., Hasegawa, O., Ulian, E.C., Peterson, G. and Smith, R.H. 1991. Transformation of Zea mays L using Agrobacterium tufemaciens and the shoot apex. Plant Physiol. 95: 426鈥?34.CAS聽 Article聽Google Scholar聽 4Shen, W.-H., Escudero, J., Schl盲ppi, M., Ramos, C., Hohn, B. and Koukolikov盲Nicola, Z. 1993. T-DNA transfer to maize cells: Histochemical investigation of 尾-glucuronidase activity in maize tissues. Proc. Natl. Acad. Sci. USA 90: 1488鈥?492.CAS聽 Article聽Google Scholar聽 5Chan, M.-T., Lee, T.-M. and Chang, H.-H. 1992. Transformation of indica rice (Oryza sativa L.) mediated by Agrobacterium tumefaciens. Plant Cell Physiol. 33: 577鈥?83.CAS聽Google Scholar聽 6Chan, M.-T., Chang, H.-H., Ho, S.-L., Tong, W.-F. and Yu, S.-M. 1993. Agrobacterium-mediated production of transgenic rice plants expessing a chimeric 伪-amylase promoter/尾-glucuronidase gene. Plant Mol. Biol. 22: 491鈥?06.CAS聽 Article聽Google Scholar聽 7Mooney, P.A., Goodwin, P.B., Dennis, E.S. and Llewellyn, D.J. 1991. Agrobacterium tumafiens-gene transfer into wheat tissues. Plant Cell Tissue Organ Cult. 25: 209鈥?18.CAS聽Google Scholar聽 8Hiei, Y., Ohta, S., Komari, T. and Kumasho, T. 1994. Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA. The Plant Journal 6: 271鈥?82.CAS聽 Article聽Google Scholar聽 9Armstrong, C.L. and Green, C.E. 1985. Established and maintenance of friable, embrygenic maize callus and the involvement of L-proline. Planta 164: 207鈥?14.CAS聽 Article聽Google Scholar聽 10Koziel, M.G. et al. 1993. Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thurigiensis . Bio/Technology 11: 194鈥?00.CAS聽Google Scholar聽 11Wan, Y., Widholdm, J.M. and Lemaux, P.G. 1995. Type I callus as a bombardment target for generating fertile transgenic maize (Zea mays L.) Planta 196: 7鈥?4.CAS聽 Article聽Google Scholar聽 12Frame, B.R., Drayton, P.R., Bagnall, S.V., Lewnau, C.J., Bullock, W.P., Wilson, H.M., Dunwell, J.M., Thompson, J.A. and Wang, K. 1994. Production of fertile transgenic maize plants by silicon carbide whisker-mediated transformation. The Plant Journal 6: 941鈥?48.CAS聽 Article聽Google Scholar聽 13D\'Halluin, K., Bonne, E., Bossut, M., Beuckeleer, M.D. and Leemans, J. 1992. Transgenic maize plants by tissue electroporation. The Plant Cell 4: 1495鈥?505.CAS聽 Article聽Google Scholar聽 14Chu, C.-C. 1978. The N6 medium and its applications to anther culture of cereal crops, pp. 43鈥?0 in Proc. Symp. Plant Tissue Culture. Science Press, Peking. Google Scholar聽 15Linsmaier, E. and Skoog, F. 1965. Organic growth factor requirements of tobacco tissue culture. Physiol. Plant. 18: 100鈥?27.CAS聽 Article聽Google Scholar聽 16Does, M.R., Dekker, B.M.M., de Groot, M.J.A., and Offringa, R. 1991. A quick method to estimate the T-DNA copy number i transgenic plants at an early stage after transformation, using inverse PCR. Plant Mol. Biol. 17: 151鈥?53.CAS聽 Article聽Google Scholar聽 17Yadav, N.S., Vanderleyden, J., Bennett, D.R., Barnes, W.M. and Chilton, M.D. 1982. Short direct repeats flank the T-DNA on a nopaline Ti plasmid. Proc. Natl. Acad. Sci. USA 79: 6322鈥?326.CAS聽 Article聽Google Scholar聽 18Zambryski, P., Depicker, A., Kruger, K. and Goodman, H.M. 1982. Tumor induction by Agrobacterium tumefaciens: analysis of the boundaries of T-DNA. J. Mol. Appl. Genet. 1: 361鈥?70.CAS聽Google Scholar聽 19Ohta, S., Mita, S., Hattori, T. and Nakamura, K. 1990. Construction and expression in tobacco of a 尾-glucuronidase (GUS) reporter gene containing an intron within the coding sequence. Plant Cell Physiol. 31: 805鈥?13.CAS聽Google Scholar聽 20Rhodes, C.A., Pierce, D.A., Mettler, I.J., Mascarenhas, D. and Detmer, J.J. 1988. Genetically transformed maize plants from protoplasts. Science 240: 204鈥?07.CAS聽 Article聽Google Scholar聽 21Gordon-Kamm, W.J. et al. 1990. Transformation of maize cells and regeneration of fertile transgenic plants. The Plant Cell 2: 603鈥?18.CAS聽 Article聽Google Scholar聽 22Fromm, M.E., Morrish, F., Armstrong, C., Williams, R., Thomas, J. and Klein, T.M. 1990. Inheritance and expression of chimeric genes in the progeny of transgenic maize plants. Bio/Technology 8: 833鈥?39.CAS聽Google Scholar聽 23Walters, D.A., Vetsch, C.S., Potts, D.E. and Lundquist, R.C. 1992. Transformation and inheritance of a hygromycin phosphotansferase gene in maize plants. Plant Mol. Biol. 18: 189鈥?00.CAS聽 Article聽Google Scholar聽 24Komari, T. 1990. Transformation of cultured cells of Chenopodium quinoa by binary vectors that carry a fragment of DNA from the virulence region of pTiBo542. Plant Cell Rep. 9: 303鈥?06.CAS聽 Article聽Google Scholar聽 25Denecke, J., Gosel茅, J., Botterman, J. and Cornelissen, M. 1989. Quantitative analysis of transiently expressed genes in plant cells. Methods in Molecular and Cellular Biology 1: 19鈥?7.CAS聽Google Scholar聽 26Ditta, G., Stanfield, S., Corbin, D. and Helinski, D.R. 1980. Broad host range DNA cloning system for Gram-negative bacteria: Construction of a gene bank of Rhizobium meliloti . Proc. Natl. Acad. Sci. USA 77: 7347鈥?351.CAS聽 Article聽Google Scholar聽 27Komari, T., Saito, Y., Nakakido, F. and Kumashiro, T. 1989. Efficient selection of somatic hybrids in Nicotiana tabacum L. using a combination of drug-resistance markers introduced by transformation. Theor. Appl. Genet. 77: 547鈥?52.CAS聽 Article聽Google Scholar聽 28Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY Google Scholar聽 29Jefferson, R.A. 1987. Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol. Biol. Rep. 5: 387鈥?05.CAS聽 Article聽Google Scholar聽 Download referencesAuthor informationAuthor notesTakashi KumashiroPresent address: Agribusiness Division, Japan Tobacco Inc., 2-1 Toranomon 2-chome, Minato-ku, Tokyo, 105, JapanYuji Ishida: KYR03634@niftyserve.or.jpAffiliationsPlant Breeding and Genetics Research Laboratory, Japan Tobacco Inc., 700 Higashibara, Iwata, Shizuoka, 438, JapanYuji Ishida,聽Hideaki Saito,聽Shozo Ohta,聽Yukoh Hiei,聽Toshihiko Komari聽 聽Takashi KumashiroAuthorsYuji IshidaView author publicationsYou can also search for this author in PubMed聽Google ScholarHideaki SaitoView author publicationsYou can also search for this author in PubMed聽Google ScholarShozo OhtaView author publicationsYou can also search for this author in PubMed聽Google ScholarYukoh HieiView author publicationsYou can also search for this author in PubMed聽Google ScholarToshihiko KomariView author publicationsYou can also search for this author in PubMed聽Google ScholarTakashi KumashiroView author publicationsYou can also search for this author in PubMed聽Google ScholarRights and permissionsReprints and PermissionsAbout this articleCite this articleIshida, Y., Saito, H., Ohta, S. et al. High efficiency transformation of maize (Zea mays L.) mediated by Agrobacterium tumefaciens. Nat Biotechnol 14, 745鈥?50 (1996). https://doi.org/10.1038/nbt0696-745Download citationReceived: 29 December 1995Accepted: 28 February 1996Issue Date: 01 June 1996DOI: https://doi.org/10.1038/nbt0696-745 Masoumeh Asadi-Aghbolaghi, Beata Dedicova, Sonali Sachi Ranade, Kim-Cuong Le, Farzad Sharifzadeh, Mansoor Omidi Ulrika Egertsdotter Plant Methods (2021) Joanna Melonek, Jorge Duarte, Jerome Martin, Laurent Beuf, Alain Murigneux, Pierrick Varenne, Jordi Comadran, Sebastien Specel, Sylvain Levadoux, Kalia Bernath-Levin, Fran莽ois Torney, Jean-Philippe Pichon, Pascual Perez Ian Small Nature Communications (2021) Albert P. Kausch, Kimberly Nelson-Vasilchik, Michael Tilelli Joel P. Hague In Vitro Cellular Developmental Biology - Plant (2021) Sign up for the Nature Briefing newsletter 鈥?what matters in science, free to your inbox daily.